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Image Search Results
Journal: Clinical and Experimental Immunology
Article Title: Multi-nucleated giant cell formation from human cord blood monocytes in vitro , in comparison with adult peripheral blood monocytes
doi: 10.1111/j.1365-2249.2009.03990.x
Figure Lengend Snippet: Morphology of multi-nucleated giant cells (MGCs) induced by interleukin (IL)-4, IL-4 with macrophage colony-stimulating factor (M-CSF) and IL-4 with granulocyte–macrophage colony-stimulating factor (GM-CSF) in vitro. Adult peripheral blood and cord blood monocytes were cultured in RPMI-1640 medium supplemented with 10% heat-inactivated fetal bovine serum (FBS), 100 U/ml penicillin and 100 µg/ml streptomycin, at 1 × 106 cells/ml/well in 24-well tissue culture plates with or without cytokines. After 14 days' incubation without changing the medium, the cells were stained with a May–Grünwald–Giemsa stain. (a) No added cytokine: adult blood; (b) no added cytokine: cord blood; (c) IL-4 (20 ng/ml) alone: adult blood; (d) IL-4 (20 ng/ml) alone: cord blood; (e) IL-4 (20 ng/ml)+M-CSF (100 ng/ml): adult blood; (f) IL-4 (20 ng/ml)+M-CSF (100 ng/ml): cord blood; (g) IL-4 (20 ng/ml)+GM-CSF (20 ng/ml): adult blood; (h) IL-4 (20 ng/ml)+GM-CSF (20 ng/ml): cord blood.
Article Snippet: The human
Techniques: In Vitro, Cell Culture, Incubation, Staining, Giemsa Stain
Journal: Clinical and Experimental Immunology
Article Title: Multi-nucleated giant cell formation from human cord blood monocytes in vitro , in comparison with adult peripheral blood monocytes
doi: 10.1111/j.1365-2249.2009.03990.x
Figure Lengend Snippet: Effect of cytokines on multi-nucleated giant cell (MGC) formation; adult blood versus cord blood monocytes. Adult peripheral blood (solid bar) and cord blood (open bar) monocytes were cultured as described in the legend to Fig. 1. The results were calculated from seven independent experiments as the fusion index. Significantly different from control or between cord blood and adult blood: *P < 0·0001, **P < 0·01, †P < 0·05.
Article Snippet: The human
Techniques: Cell Culture
Journal: Clinical and Experimental Immunology
Article Title: Multi-nucleated giant cell formation from human cord blood monocytes in vitro , in comparison with adult peripheral blood monocytes
doi: 10.1111/j.1365-2249.2009.03990.x
Figure Lengend Snippet: Nuclei per multi-nucleated giant cells (MGCs) induced by cytokines; adult blood versus cord blood monocytes. Adult peripheral blood (solid box) and cord blood (open box) monocytes were cultured as described in the legend to Fig. 1. The number of nuclei per MGC (20 cells acquired randomly in each experiment) was counted. Results from seven independent experiments are shown in the box-and-whisker plots. Line in the box shows median point, box shows the point from first to third quartile, whiskers show the point from smallest to largest number. Significantly different between cord blood and adult blood: *P < 0·0001.
Article Snippet: The human
Techniques: Cell Culture, Whisker Assay
Journal: Clinical and Experimental Immunology
Article Title: Multi-nucleated giant cell formation from human cord blood monocytes in vitro , in comparison with adult peripheral blood monocytes
doi: 10.1111/j.1365-2249.2009.03990.x
Figure Lengend Snippet: Concentration of interferon (IFN)-γ, interleukin (IL)-1β, IL-4 and tumour necrosis factor (TNF)-α in the supernatants of adult peripheral blood and cord blood monocytes after 14 days' culture with the cytokines listed below.
Article Snippet: The human
Techniques: Concentration Assay